From d029d5d7f8ead1f1de8d318045004a4a6f68f5fb Mon Sep 17 00:00:00 2001 From: Bonface Date: Fri, 9 Feb 2024 09:41:28 -0600 Subject: Update dataset RTF Files. --- .../DoDTATRCRetBLMoGene2_1213/acknowledgment.rtf | 1 + .../datasets/DoDTATRCRetBLMoGene2_1213/cases.rtf | 1728 ++++++++++++++++++++ .../DoDTATRCRetBLMoGene2_1213/platform.rtf | 1 + .../DoDTATRCRetBLMoGene2_1213/processing.rtf | 1 + .../datasets/DoDTATRCRetBLMoGene2_1213/summary.rtf | 1 + .../datasets/DoDTATRCRetBLMoGene2_1213/tissue.rtf | 1 + 6 files changed, 1733 insertions(+) create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/acknowledgment.rtf create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/cases.rtf create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/platform.rtf create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/processing.rtf create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/summary.rtf create mode 100644 general/datasets/DoDTATRCRetBLMoGene2_1213/tissue.rtf (limited to 'general/datasets/DoDTATRCRetBLMoGene2_1213') diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/acknowledgment.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/acknowledgment.rtf new file mode 100644 index 0000000..13c6b1e --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/acknowledgment.rtf @@ -0,0 +1 @@ +

DoD funding (W81XWH-12-0255) Genetic Networks Activated by Blast Injury to the Eye (Eldon E. Geisert).

diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/cases.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/cases.rtf new file mode 100644 index 0000000..6b36c70 --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/cases.rtf @@ -0,0 +1,1728 @@ +

Almost all animals are young adults between 60 and 90 days of age (Table 1, minimum age is 50 and maximum age is 134 days). We measured expression in conventional inbred strains, BXD recombinant inbred (RI) strains, and reciprocal F1s between C57BL/6J and DBA/2J. BXD strains: The first 32 of these strains are from the Taylor series of BXD strains generated at the Jackson Laboratory by Benjamin A. Taylor. BXD1 through BXD32 were started in the late 1970s, whereas BXD33 through 42 were started in the 1990s. In 2004, BXD24/TyJ developed a spontaneous mutation, rd16 which resulted in retinal degeneration and was renamed BXD24b/TyJ (BXD24 in this database). The strain, BXD24a, was cryo-recovered in 2004 from 1988 embryo stocks (F80) and does not exhibit retinal degeneration. In 2009, BXD24b was renamed BXD24/TyJ-Cep290rd16/J by JAX Labs to reflect the discovery of the genetic basis of the mutation. At the same time BXD24a was then referred to just as BXD24/TyJ by Jax Labs, but still called BXD24a in this dataset. The other 36 BXD strains (BXD43 and higher) were bred by Lu Lu, Jeremy Peirce, Lee M. Silver, and Robert W. Williams starting in 1997 using B6D2 generation 10 advanced intercross progeny. This modified breeding protocol doubles the number of recombinations per BXD strain and improves mapping resolution (Peirce et al. 2004). All of the Taylor series of BXD strains and many of the new BXD strains are available from the Jackson Laboratory. All of the new BXD strains (BXD43 and higher) are also available directly from Lu Lu and colleagues at the University of Tennessee Health Science Center in Memphis, TN, USA. BXD24/TyJ is now known as BXD24b/TyJ and has nearly complete retinal degeneration. BXD24a/TyJ, a 1988 F80 inbred stock that has been rederived from cryogenic storage, does not have retinal degeneration (stock number 005243) and is an ideal coisogenic control, but is not included in the HEI data set.

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IndexEIGC ID (LIMS ID)StrainSexAge at harvestRIN Score
1602BXD9F1339.1
2603BXD9M1009.5
3604BXD40F1009.4
4605BXD40M1009.1
5606BXD48F759.1
6607BXD48M758.6
7608BXD63F759.2
8609BXD63M619.4
9612BXD73M10810
10614BXD87M859.2
11615BXD87F859.9
12616BXD69F769.3
13617BXD69M10010
14618BXD51M828.9
15619BXD92 (BXD65b)M828.8
16620BXD92 (BXD65b)F828.8
17635BXD100M709
18636BXD100M708.8
19652BXD92 (BXD65b)F649
20653BXD87F679.7
21654BXD87M678.9
22655BXD63M719
23660BXD69M9710
24687BXD6M857.7
25688BXD6M859.4
26690BXD12F8310
27691BXD12F8310
28692BXD12M8310
29695BXD5F7710
30696BXD5M7710
31698BXD5F779.9
32699BXD5F7710
33701BXD8M7910
34702BXD8M7910
35705BXD8F779.2
36707BXD15F779.4
37708BXD15F779.1
38710BXD15M779.1
39713BXD22F719.5
40714BXD22F719.7
41716BXD22M719.1
42717BXD22M719.1
43719BXD14F709.2
44722BXD14M708.9
45723BXD14M709.2
46725BXD18F709
47726BXD18F709.1
48728BXD18M709.1
49729BXD18M709.2
50731BXD19F669.3
51732BXD19F669.4
52734BXD19M669.8
53735BXD19M669.4
54737BXD21F719.7
55738BXD21F719.6
56740BXD21M719.1
57741BXD21M719.7
58743BXD2F708.3
59744BXD2F708.6
60746BXD2M707.5
61747BXD2M778.6
62768BXD48M1219.4
63770BXD92 (BXD65b)M6510
64771BXD101F858.3
65772BXD101F859.3
66773BXD101M869.5
67774BXD101M869.8
68775BXD102F669.5
69776BXD102F6610
70777BXD102M849.6
71787BXD60M869.9
72788BXD60M869.9
73789BXD63F1159.7
74790BXD100F729.8
75791BXD100F7210
76818BXD51F779.7
77819BXD51F779.6
78820BXD51M729.7
79821BXD65M729.6
80822BXD65M659.5
81825BXD65F729.5
82827BXD86M699.1
83828BXD86M699.4
84829BXD90F699.6
85831BXD9M1279.7
86832BXD39F999.4
87833BXD39F1079.3
88834BXD60F1079.5
89836BXD84M1039.7
90846BXD39M639.7
91847BXD39M639.8
92849BXD20F7010
93850BXD20M7010
94851BXD20*709.5
95855DBA/2JF789.7
96856DBA/2JF7810
97857DBA/2JM7810
98858DBA/2JM789.9
99859C57BL/6JF7810
100860C57BL/6JF7810
101861C57BL/6JM7810
102862C57BL/6JM789.6
103EGE10907-0172_890_20844BXD28M7510
104EGE10907-0173_891_20845BXD28M759.7
105EGE10907-0174_892_20846BXD28F759.7
106EGE10907-0176_895_20848BXD33M759.88
107EGE10907-0177_896_20849BXD33F7510
108EGE10907-0178_897_20850BXD33F759.4
109EGE10907-0179_898_20851BXD36M759.4
110EGE10907-0180_899_20852BXD36F759.5
111EGE10907-0181_900_20853BXD36F759.1
112EGE10907-0182_910_20854BXD27M709.7
113EGE10907-0183_990_20855BXD9F989.4
114EGE10907-0184_991_20856BXD16F11310
115EGE10907-0185_992_20857BXD16M1139
116EGE10907-0186_993_20858BXD27F969.4
117EGE10907-0187_994_20859BXD27F969.4
118EGE10907-0188_996_20860BXD48F989.4
119EGE10907-0189_997_20861BXD73M10610
120EGE10907-0190_999_20862BXD11F1219.6
121EGE10907-0191_100_20863BXD11F1219.9
122EGE10907-0192_1010_21276BXD13M699.8
123EGE10907-0193_1011_21277BXD13M699.8
124EGE10907-0194_1012_21278BXD16F6510
125EGE10907-0195_1015_21279BXD16M6510
126EGE10907-0196_1019_21280BXD33M7510
127EGE10907-0197_1020_21281BXD36M7810
128EGE10907-0198_1021_21282BXD38F709.8
129EGE10907-0199_1022_21283BXD38F7010
130EGE10907-0200_1023_21284BXD38M709.7
131EGE10907-0201_1047_21285BXD11M6510
132EGE10907-0202_1048_21286BXD11M6510
133EGE10907-0203_1049_21287BXD38M7710
134EGE10907-0204_1050_21288BXD73F7010
135EGE10907-0205_1051_21289BXD73F7010
136EGE10907-0206_1052_21290BXD86F6910
137EGE10907-0207_1053_21291BXD86F6910
138EGE10907-0208_1054_21292BXD90F7110
139EGE10907-0209_1055_21293BXD90M7110
140EGE10907-0210_1056_21294BXD90M7110
141EGE10907-0211_1148_21295BXD31F9010
142EGE10907-0212_1149_21296BXD31F9010
143EGE10907-0213_1150_21297BXD31M9010
144EGE10907-0214_1151_21298BXD31M909.9
145EGE10907-0215_1152_21299BXD42F8310
146EGE10907-0216_21429BXD42F8310
147EGE10907-0217_21430BXD42M8310
148EGE10907-0218_21431BXD42M839.3
149EGE10907-0219_21432BXD50F929.6
150EGE10907-0220_21433BXD50F929.6
151EGE10907-0221_21434BXD50F9210
152EGE10907-0222_21435BXD1M739.6
153EGE10907-0223_21436BXD13F719.7
154EGE10907-0224_21437BXD1M719.5
155EGE10907-0225_21438BXD13F719.6
156EGE10907-0226_21439BXD43F8510
157EGE10907-0227_21452BXD43F859.6
158EGE10907-0229_21442BXD43M858.2
159EGE10907-0230_21443BXD50M699.4
160EGE10907-0231_21444BXD1F689.4
161EGE10907-0232_21445BXD1F689.6
162EGE10907-0233_21446BXD29M659.5
163EGE10907-0234_21447BXD29M6510
164EGE10907-0235_21448BXD75F6810
165EGE10907-0236_21449BXD75M6810
166EGE10907-0237_21450BXD96F679.9
167EGE10907-0238_21931BXD99M717.9
168EGE10907-0239_21932BXD99M719.7
169EGE10907-0240_21933BXD67F688.8
170EGE10907-0241_21934BXD67F688.5
171EGE10907-0242_21935BXD67M689.5
172EGE10907-0243_21936BXD67M689.6
173EGE10907-0244_21937BXD6F689.6
174EGE10907-0245_21938BXD12M829.5
175EGE10907-0246_21939BXD60F729.6
176EGE10907-0247_21940BXD65F759.1
177EGE10907-0248_21941BXD75F698.8
178EGE10907-0249_21942_(MoGene-2_0-st)BXD75M699.2
179EGE10907-0250_21943BXD8F979.1
180EGE10907-0251_21944BXD85F798.6
181EGE10907-0252_21945BXD85M799.1
182EGE10907-0253_21946BXD85M799.4
183EGE10907-0254_21947BXD102M948.6
184EGE10907-0255_21948BXD99F839
185EGE10907-0256_21949BXD69F689
186EGE10907-0257_21950BXD84F749.5
187EGE10907-0258_21951BXD84F749.4
188EGE10907-0259_21952BXD84M748.6
189EGE10907-0260_21451BXD96M679.2
190EGE10907-0261_21452BXD99F718.9
191EGE10907-0262_21931BXD6F669.4
192EGE10907-0263_21932BXD14F698.5
193EGE10907-0264_21933BXD34M799.1
194EGE10907-0265_21934BXD34M799.9
195EGE10907-0266_21935BXD40F759.6
196EGE10907-0267_21936BXD40M789.7
197EGE10907-0268_21937BXD56F729.7
198EGE10907-0269_21938BXD56F729.7
199EGE10907-0270_21939BXD56M729.6
200EGE10907-0271_21940BXD56M729.4
201EGE10907-0272_21941BXD71F729.9
202EGE10907-0273_21942BXD71F759.4
203EGE10907-0274_21943BXD71M7510
204EGE10907-0275_21944BXD71M7510
205EGE10907-0276_21945BXD27M6510
206EGE10907-0277_21946BXD15M6510
207EGE10907-0278_21947BXD20F12510
208EGE10907-0279_21948BXD29F7710
209EGE10907-0280_21949BXD29F7710
210EGE10907-0281_21950BXD34F7410
211EGE10907-0282_21951BXD34F7410
212EGE10907-0283_21952BXD85F8710
213EGE10907-0284_21451BXD43M759.8
+
+
diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/platform.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/platform.rtf new file mode 100644 index 0000000..19fc38c --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/platform.rtf @@ -0,0 +1 @@ +

The Affymetrics GeneChip® Mouse Gene 2.0 ST microarrays. This relatively recent microarray was specifically designed to represent the whole-transcriptome. It includes probes to measure mRNA, long intergenic non-coding RNAs and microRNAs. Researchers have identified many transcripts in the mouse genome that do not have protein coding potential. Most of these non-coding RNAs have little functional annotation. Nonetheless, there is considerable evidence that these non-coding RNAs play important roles in development of the retina and the progression of disease. The coverage in the new ST 2.0 array includes over 28,000 coding transcripts and over 7,000 non-coding transcripts. There are also probes on the array covering 590 microRNAs.

diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/processing.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/processing.rtf new file mode 100644 index 0000000..a7b767e --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/processing.rtf @@ -0,0 +1 @@ +

Replication, sex, and sample balance: Our goal was to obtain data for independent biological sample pools from both sexes for most lines of mice. The four batches of arrays included in this final data set, collectively represent a reasonably well-balanced sample of males and females, in general without within-strain-by-sex replication.

diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/summary.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/summary.rtf new file mode 100644 index 0000000..96a87e5 --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/summary.rtf @@ -0,0 +1 @@ +

The DoD dataset was constructed using the Affymetrics GeneChip® Mouse Gene 2.0 ST microarrays. This relatively recent microarray was specifically designed to represent the whole-transcriptome. It includes probes to measure mRNA, long intergenic non-coding RNAs and microRNAs. Researchers have identified many transcripts in the mouse genome that do not have protein coding potential. Most of these non-coding RNAs have little functional annotation. There is considerable evidence that these non-coding RNAs play important roles in development of the retina and the progression of disease. The coverage in the new ST 2.0 array includes over 28,000 coding transcripts and over 7,000 non-coding transcripts. There are also probes on the array covering 590 microRNAs. This is RMA expression data that has been normalized using what we call a 2z+8 scale, but without special correction for batch effects. The data for each strains were computed as the mean of four samples per strain. Expression values on a log2 scale range from 3.77 to 14.62, a nominal range of approximately 3600-fold. After taking the log2 of the original non-logged expression estimates, we convert data within an array to a z score. We then multiply the z score by 2. Finally, we add 8 units to ensure that no values are negative. The result is a scale with a mean of 8 units and a standard deviation of 2 units. A two-fold difference in expression is equivalent roughly to 1 unit on this scale.

diff --git a/general/datasets/DoDTATRCRetBLMoGene2_1213/tissue.rtf b/general/datasets/DoDTATRCRetBLMoGene2_1213/tissue.rtf new file mode 100644 index 0000000..056d7eb --- /dev/null +++ b/general/datasets/DoDTATRCRetBLMoGene2_1213/tissue.rtf @@ -0,0 +1 @@ +

Tissue preparation protocol. Animal were killed by rapid cervical dislocation. Retinas were removed immediately and placed in RiboLock (RiboLock: Thermo Scientific RiboLock RNase #EO0381 40U/µl 2500U) at room temperature. Individual retinas from the mouse were stored in each tube. RNA was isolated using a Qiacube with the resultant RIN scores ranging from 8.5 to 10.0.

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