From d029d5d7f8ead1f1de8d318045004a4a6f68f5fb Mon Sep 17 00:00:00 2001 From: Bonface Date: Fri, 9 Feb 2024 09:41:28 -0600 Subject: Update dataset RTF Files. --- general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf | 1 + 1 file changed, 1 insertion(+) create mode 100644 general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf (limited to 'general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf') diff --git a/general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf b/general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf new file mode 100644 index 0000000..aee498f --- /dev/null +++ b/general/datasets/DevStriatum_ILM6_2P3RInv_1110/processing.rtf @@ -0,0 +1 @@ +

Samples were processed by Lorne Rose and colleagues in the Illumina Core at UTHSC between July and August 2010. In brief, RNA purity was evaluated using the 260/280 nm absorbance ratio, and values had to be greater than 1.8 to pass our quality control (QC). The majority of samples had values between 1.9 and 2.1. RNA integrity was assessed using the Agilent Bioanalyzer 2100. The standard Eberwine T7 polymerase method was used to catalyze the synthesis of cDNA template from polyA-tailed RNA using the Ambion/Illumina (http://www.ambion.com/catalog/CatNum.php?AMIL1791) TotalPrep RNA amplication kit (Cat IL1791). The biotin labeled cRNA was then evaluated using both the 260/280 ratio (values of 2.0-2.3 are acceptable) using a NanoDrop ND-1000 (http://www.nanodrop.com/nd-1000-overview.html). Those samples that passed QC steps (1-3% failed and new RNA samples had to be acquired and processed) were immediately used on Mouse-6 v 1.1 slide. The slides were hybridized and washed following standard Illumina protocols.

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