From d029d5d7f8ead1f1de8d318045004a4a6f68f5fb Mon Sep 17 00:00:00 2001 From: Bonface Date: Fri, 9 Feb 2024 09:41:28 -0600 Subject: Update dataset RTF Files. --- general/datasets/BR_M2_1106_R/tissue.rtf | 2751 ++++++++++++++++++++++++++++++ 1 file changed, 2751 insertions(+) create mode 100644 general/datasets/BR_M2_1106_R/tissue.rtf (limited to 'general/datasets/BR_M2_1106_R/tissue.rtf') diff --git a/general/datasets/BR_M2_1106_R/tissue.rtf b/general/datasets/BR_M2_1106_R/tissue.rtf new file mode 100644 index 0000000..0989cd0 --- /dev/null +++ b/general/datasets/BR_M2_1106_R/tissue.rtf @@ -0,0 +1,2751 @@ +
+-- cgit v1.2.3Naïve male mice were euthanized by CO2 exposure, and whole brains were removed and frozen on dry ice. Brains were stored at -70 deg C until used. The RNeasy Midi kit for lipid-rich tissues (Qiagen, Valencia, CA) was used to extract total RNA, and the RNeasy Mini kit (Qiagen) was used for cleanup. Biotin-labeled cRNA was obtained by in vitro transcription of the double-stranded cDNA that was originally synthesized from the total RNA. Each whole brain sample of biotin-labeled cRNA was fragmented and hybridized to a separate oligonucleotide array. After hybridization, the chips were stained with streptavidin-phycoerythrin conjugate and scanned using an Affymetrix GeneArray scanner.
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