""" This module contains functions relating to specific trait dataset manipulation """ import json from typing import Any from pathlib import Path from pymonad.either import Either, Left, Right from flask import current_app as app from gn3.commands import monadic_run_cmd def retrieve_sample_list(group: str, inc_par: bool = True, inc_f1: bool = True): """ Get the sample list for a group (a category that datasets belong to) Currently it is fetched from the .geno files, since that's the only place the "official" sample list is stored """ samplelist = [] if inc_par or inc_f1: par_f1_path = Path( app.config.get( "GENENETWORK_FILES", "/home/gn2/production/genotype_files/" ), 'parents_and_f1s.json' ) if par_f1_path.is_file(): with open(par_f1_path, encoding="utf-8") as par_f1_file: par_f1s = json.load(par_f1_file).get(group, {}) if inc_par and par_f1s: samplelist += [par_f1s['paternal']['strain'], par_f1s['maternal']['strain']] if inc_f1 and par_f1s: samplelist += [f1['strain'] for f1 in par_f1s['f1s']] genofile_path = Path( app.config.get( "GENENETWORK_FILES", "/home/gn2/production/genotype_files/" ), f'genotype/{group}.geno' ) if genofile_path.is_file(): with open(genofile_path, encoding="utf-8") as genofile: line = "" for line in genofile: line = line.strip() if not line: continue if line.startswith(("#", "@")): continue break headers = line.split("\t") if headers[3] == "Mb": samplelist += headers[4:] else: samplelist += headers[3:] return samplelist def retrieve_mrna_group_name(connection: Any, probeset_id: int, dataset_name: str): """ Given the trait id (ProbeSet.Id in the database), retrieve the name of the group the dataset belongs to. """ query = ( "SELECT iset.Name " "FROM ProbeSet ps LEFT JOIN ProbeSetXRef psx ON psx.ProbeSetId = ps.Id " "LEFT JOIN ProbeSetFreeze psf ON psx.ProbeSetFreezeId = psf.Id " "LEFT JOIN ProbeFreeze pf ON psf.ProbeFreezeId = pf.Id " "LEFT JOIN InbredSet iset ON pf.InbredSetId = iset.Id " "WHERE ps.Id = %(probeset_id)s AND psf.Name=%(dataset_name)s") with connection.cursor() as cursor: cursor.execute(query, {"probeset_id": probeset_id, "dataset_name": dataset_name}) res = cursor.fetchone() if res: return res[0] return None def retrieve_phenotype_group_name(connection: Any, dataset_id: int): """ Given the dataset id (PublishFreeze.Id in the database), retrieve the name of the group the dataset belongs to. """ query = ( "SELECT iset.Name " "FROM InbredSet AS iset " "WHERE iset.Id = %(dataset_id)s") with connection.cursor() as cursor: cursor.execute(query, {"dataset_id": dataset_id}) res = cursor.fetchone() if res: return res[0] return None def retrieve_probeset_trait_dataset_name( threshold: int, name: str, connection: Any): """ Get the ID, DataScale and various name formats for a `ProbeSet` trait. """ query = ( "SELECT Id, Name, FullName, ShortName, DataScale " "FROM ProbeSetFreeze " "WHERE " "public > %(threshold)s " "AND " "(Name = %(name)s OR FullName = %(name)s OR ShortName = %(name)s)") with connection.cursor() as cursor: cursor.execute( query, { "threshold": threshold, "name": name }) res = cursor.fetchone() if res: return dict(zip( ["dataset_id", "dataset_name", "dataset_fullname", "dataset_shortname", "dataset_datascale"], res)) return {"dataset_id": None, "dataset_name": name, "dataset_fullname": name} def retrieve_publish_trait_dataset_name( threshold: int, name: str, connection: Any): """ Get the ID, DataScale and various name formats for a `Publish` trait. """ query = ( "SELECT Id, Name, FullName, ShortName " "FROM PublishFreeze " "WHERE " "public > %(threshold)s " "AND " "(Name = %(name)s OR FullName = %(name)s OR ShortName = %(name)s)") with connection.cursor() as cursor: cursor.execute( query, { "threshold": threshold, "name": name }) return dict(zip( ["dataset_id", "dataset_name", "dataset_fullname", "dataset_shortname"], cursor.fetchone())) def retrieve_geno_trait_dataset_name( threshold: int, name: str, connection: Any): """ Get the ID, DataScale and various name formats for a `Geno` trait. """ query = ( "SELECT Id, Name, FullName, ShortName " "FROM GenoFreeze " "WHERE " "public > %(threshold)s " "AND " "(Name = %(name)s OR FullName = %(name)s OR ShortName = %(name)s)") with connection.cursor() as cursor: cursor.execute( query, { "threshold": threshold, "name": name }) return dict(zip( ["dataset_id", "dataset_name", "dataset_fullname", "dataset_shortname"], cursor.fetchone())) def retrieve_dataset_name( trait_type: str, threshold: int, dataset_name: str, conn: Any): """ Retrieve the name of a trait given the trait's name This is extracted from the `webqtlDataset.retrieveName` function as is implemented at https://github.com/genenetwork/genenetwork1/blob/master/web/webqtl/base/webqtlDataset.py#L140-L169 """ fn_map = { "ProbeSet": retrieve_probeset_trait_dataset_name, "Publish": retrieve_publish_trait_dataset_name, "Geno": retrieve_geno_trait_dataset_name, "Temp": lambda threshold, dataset_name, conn: {}} return fn_map[trait_type](threshold, dataset_name, conn) def retrieve_geno_group_fields(name, conn): """ Retrieve the Group, and GroupID values for various Geno trait types. """ query = ( "SELECT InbredSet.Name, InbredSet.Id " "FROM InbredSet, GenoFreeze " "WHERE GenoFreeze.InbredSetId = InbredSet.Id " "AND GenoFreeze.Name = %(name)s") with conn.cursor() as cursor: cursor.execute(query, {"name": name}) return dict(zip(["group", "groupid"], cursor.fetchone())) return {} def retrieve_publish_group_fields(name, conn): """ Retrieve the Group, and GroupID values for various Publish trait types. """ query = ( "SELECT InbredSet.Name, InbredSet.Id " "FROM InbredSet, PublishFreeze " "WHERE PublishFreeze.InbredSetId = InbredSet.Id " "AND PublishFreeze.Name = %(name)s") with conn.cursor() as cursor: cursor.execute(query, {"name": name}) return dict(zip(["group", "groupid"], cursor.fetchone())) return {} def retrieve_probeset_group_fields(name, conn): """ Retrieve the Group, and GroupID values for various ProbeSet trait types. """ query = ( "SELECT InbredSet.Name, InbredSet.Id " "FROM InbredSet, ProbeSetFreeze, ProbeFreeze " "WHERE ProbeFreeze.InbredSetId = InbredSet.Id " "AND ProbeFreeze.Id = ProbeSetFreeze.ProbeFreezeId " "AND ProbeSetFreeze.Name = %(name)s") with conn.cursor() as cursor: cursor.execute(query, {"name": name}) return dict(zip(["group", "groupid"], cursor.fetchone())) return {} def retrieve_temp_group_fields(name, conn): """ Retrieve the Group, and GroupID values for `Temp` trait types. """ query = ( "SELECT InbredSet.Name, InbredSet.Id " "FROM InbredSet, Temp " "WHERE Temp.InbredSetId = InbredSet.Id " "AND Temp.Name = %(name)s") with conn.cursor() as cursor: cursor.execute(query, {"name": name}) return dict(zip(["group", "groupid"], cursor.fetchone())) return {} def retrieve_group_fields(trait_type, trait_name, dataset_info, conn): """ Retrieve the Group, and GroupID values for various trait types. """ group_fns_map = { "Geno": retrieve_geno_group_fields, "Publish": retrieve_publish_group_fields, "ProbeSet": retrieve_probeset_group_fields } if trait_type == "Temp": group_info = retrieve_temp_group_fields(trait_name, conn) else: group_info = group_fns_map[trait_type](dataset_info["dataset_name"], conn) return { **dataset_info, **group_info, "group": ( "BXD" if group_info.get("group") == "BXD300" else group_info.get("group", "")) } def retrieve_temp_trait_dataset(): """ Retrieve the dataset that relates to `Temp` traits """ return { "searchfield": ["name", "description"], "disfield": ["name", "description"], "type": "Temp", "dataset_id": 1, "fullname": "Temporary Storage", "shortname": "Temp" } def retrieve_geno_trait_dataset(): """ Retrieve the dataset that relates to `Geno` traits """ return { "searchfield": ["name", "chr"], "disfield": ["name", "chr", "mb", "source2", "sequence"], "type": "Geno" } def retrieve_publish_trait_dataset(): """ Retrieve the dataset that relates to `Publish` traits """ return { "searchfield": [ "name", "post_publication_description", "abstract", "title", "authors"], "disfield": [ "name", "pubmed_id", "pre_publication_description", "post_publication_description", "original_description", "pre_publication_abbreviation", "post_publication_abbreviation", "lab_code", "submitter", "owner", "authorized_users", "authors", "title", "abstract", "journal", "volume", "pages", "month", "year", "sequence", "units", "comments"], "type": "Publish" } def retrieve_probeset_trait_dataset(): """ Retrieve the dataset that relates to `ProbeSet` traits """ return { "searchfield": [ "name", "description", "probe_target_description", "symbol", "alias", "genbankid", "unigeneid", "omim", "refseq_transcriptid", "probe_set_specificity", "probe_set_blat_score"], "disfield": [ "name", "symbol", "description", "probe_target_description", "chr", "mb", "alias", "geneid", "genbankid", "unigeneid", "omim", "refseq_transcriptid", "blatseq", "targetseq", "chipid", "comments", "strand_probe", "strand_gene", "probe_set_target_region", "proteinid", "probe_set_specificity", "probe_set_blat_score", "probe_set_blat_mb_start", "probe_set_blat_mb_end", "probe_set_strand", "probe_set_note_by_rw", "flag"], "type": "ProbeSet" } def retrieve_trait_dataset(trait_type, trait, threshold, conn): """ Retrieve the dataset that relates to a specific trait. """ dataset_fns = { "Temp": retrieve_temp_trait_dataset, "Geno": retrieve_geno_trait_dataset, "Publish": retrieve_publish_trait_dataset, "ProbeSet": retrieve_probeset_trait_dataset } dataset_name_info = { "dataset_id": None, "dataset_name": trait["db"]["dataset_name"], **retrieve_dataset_name( trait_type, threshold, trait["db"]["dataset_name"], conn) } group = retrieve_group_fields( trait_type, trait["trait_name"], dataset_name_info, conn) return { "display_name": dataset_name_info["dataset_name"], **dataset_name_info, **dataset_fns[trait_type](), **group } def retrieve_metadata(name: str) -> dict: """Return the full data given a path, NAME""" result = {} __subject = { "summary": "description", "tissue": "tissueInfo", "specifics": "specifics", "cases": "caseInfo", "platform": "platformInfo", "processing": "processingInfo", "notes": "notes", "experiment-design": "experimentDesignInfo", "acknowledgment": "acknowledgement", "citation": "citation", "experiment-type": "experimentType", "contributors": "contributors", } for __file in Path(name).glob("*rtf"): with __file.open() as _f: result[__subject.get(__file.stem, f"gn:{__file.stem}")] = _f.read() return result def save_metadata( git_dir: str, output: str, author: str, content: str, msg: str ) -> Either: """Save dataset metadata to git""" def __write__(): try: with Path(output).open(mode="w", encoding="utf8") as file_: file_.write(content) return Right(0) except PermissionError as excpt: return Left({ "command": "Permission required to write to this file!", "error": str(excpt) }) except IOError as excpt: return Left({ "command": "IOError when writing to this file!", "error": str(excpt) }) return ( monadic_run_cmd(f"git -C {git_dir} reset --hard origin".split(" ")) .then(lambda _: monadic_run_cmd(f"git -C {git_dir} pull".split(" "))) .then(lambda _: __write__()) .then(lambda _: monadic_run_cmd(f"git -C {git_dir} add .".split(" "))) .then(lambda _: monadic_run_cmd( f"git -C {git_dir} commit -m".split(" ") + [ f'{msg}', f"--author='{author}'", "--no-gpg-sign" ])) .then(lambda _: monadic_run_cmd(f"git -C {git_dir} \ push origin master --dry-run".split(" ")))) def get_history(git_dir: str, name: str) -> Either: """Get the git history for a given dataset. This history is returned as a HTML formatted text. Example of the formatted output: ```