<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script> if ( ! top.PPTPRESENTATION ) { window.location.replace( "endshow.htm" ); } </script> <meta name=Description content="Jun-19-03: WebQTL link to www.webqtl.org/search.html"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!-- gId="slide0026.htm" if ( !parent.base.g_done && (parent.base.msie < 0 ) ) { parent.base.g_done = 1; document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref); 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height:100.0%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:68%'><i>WebQTL<span style="mso-spacerun: yes"> </span><br> </i></span></span><span style='position: absolute;top:32.43%;left:4.37%;width:95.76%'><span style='font-size:68%'><i>link to <br> </i></span></span><span style='position:absolute;top:58.55%;left:4.37%; width:95.76%'><span style='font-size:86%'><i>www.webqtl.org/search.html</i></span><span style='font-size:68%;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div style='position:absolute;top:36.57%;left:2.51%;width:73.24%;height:45.05%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='position:absolute;top:0%;left:2.71%;width:97.28%; height:81.96%'> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:0%;width:94.6%;height:31.57%'><span style='position:absolute;top:0%;left:7.46%;width:92.53%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,1";color:#FBFDB8;position:absolute;left:-8.06%;font-family:Times; font-size:99%'>1.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering shared expression </span></span><span style='position:absolute;top:43.93%;left:7.46%;width:82.12%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>patterns<br> </span></span></div> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:41.14%;left:0%;width:84.94%;height:31.57%'><span style='position:absolute;top:0%;left:8.31%;width:91.46%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,2";color:#FBFDB8;position:absolute;left:-9.09%;font-family:Times; font-size:99%'>2.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering upstream </span></span><span style='position: absolute;top:43.93%;left:8.31%;width:91.46%'><span style='font-size:94%; color:#E9EB5D;mso-color-index:3'>modulators (QTLs)<br> </span></span></div> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:82.29%;left:0%;width:100.0%;height:17.7%'><span style='position:absolute;top:0%;left:7.06%;width:92.75%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,3";color:#FBFDB8;position:absolute;left:-7.61%;font-family:Times; font-size:99%'>3.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering downstream targets</span></span></div> </div> </div> <div style='position:absolute;top:82.15%;left:4.1%;width:96.42%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> <img border=0 src="slide0026_image107.gif" style='position:absolute;top:65.9%; left:65.29%;width:26.35%;height:10.77%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Part 3.<span style="mso-spacerun: yes"> </span>Many investigators would like to discover the set of downstream targets of a gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>In a genetic and functional sense, that question can only be addressed effectively if there is genetic variation in the particular gene.<span style="mso-spacerun: yes"> </span>We know that Fos is an important transcription factor, but unless it is polymorphic between C57BL/6J and DBA/2J, then it cannot generate a genetic variance signal with which we can work. We can still study covariance of Fos and hundreds of other transcripts (an interesting exercise), but there are no genetic causes-and-effects.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!-- function playList() { } //--> </script> </body> </html>