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<P class="title">Virginia Commonwealth University LXS Prefrontal Cortex Ethanol vs Saline M430A 2.0 (Aug06) Sscore data set by Michael F. Miles and colleagues <A HREF="/webqtl/main.py?FormID=editHtml">
<img src="/images/modify.gif" alt="modify this page" border= 0 valign="middle"></A><BR><BR>Accession number: <A HREF="/webqtl/main.py?FormID=sharinginfo&GN_AccessionId=132">GN132</A></P>




<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;Summary:</P>


<blockquote>
VCU LXS PFC Et vs Sal M430A 2.0 (Aug06) Sscore DATA SET: This LXS data set provides estimates of ethanol-responsive differences in mRNA expression in the prefrontal cortex of 43 LXS recombinant inbred strains generated by crossing
ILS/Ibg and ISS/Ibg (Inbred Long Sleep and Inbred Short Sleep strains from the <A HREF="http://ibgwww.colorado.edu/" target="_empty" class="fs14">Institute of Behavioral Genetics</A>). All samples are from a total of 376 adult male animals raised in a standard laboratory environment. An average of 8 males per strain were used for basal or post-saline injection activity measurements on days 1-2, respectively.  4 animals of each strain were then injected IP with saline or ethanol (1.8 g/kg) on day 3 and locomotor activity measured for 15 minutes prior to returning to home cages.  4 hours after testing, animals were rapidly sacrificed by cervical dislocation and brains removed, cooled  and microdissected as described previously (Kerns et al., J. Neurosci. 25:2255, 2005).



<P>RNA was isolated from pools of 3-4 animals per strain/treatment group.  All RNA isolation and subsequent probe generation and hybridization to microarrays was done using a supervised randomization procedure to minimize batch effects. <A HREF="http://www.illumina.com/gex-mouse/" target="_empty" class="fs14">Affymetrix M430A 2.0</A> microarrays were used for hybridization using standard procedures. A total of 22626 estimates of expression (some of which are controls) were transformed using the S-score method to compare ethanol vs. saline expression from pairs of arrays for each strain (Kerns et al., Methods 31:274, 2003, ). The S-score is a method developed for Affymetrix oligonucleotide arrays that is particularly suited to comparing expression on 2 or a small number of chips The S-score output for each probeset is not an indication of expression magnitude but rather, the change in expression between compared arrays.  The S-score is essentially a z-score centered around zero with positive S-scores reflecting increased gene expression with ethanol and negative scores reflecting ethanol-induced decreases in expression.  Larger magnitude S-scores show more significant changes in expression and are generally correlated with the "fold-change".

<P>As a measure of data quality we often count the number of probes that are associated with higher LOD scores. In this data set, 91 probes have LRS values >23 (LOD >5). The maximum LRS achieved in this data set is 36.63 for probe set 1460695_at (<I> 2300006M17Rik</I>).


<P>
<IMG src="/images/upload/LXS_SscrMaxLOD.gif" valign="top">


<DIR><P><SMALL><B>Legend:</B> Bar chart of the expression of <I>2300006M17Rik</I> probe set 1460695_at in the LXS PFC Et vs Sal Sscore data set. This probe is associated with a LOD score of 7.96 (LRS 36.63).  </SMALL></P>
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<BLOCKQUOTE>

</Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;About the strains used to generate this set of data:</P>

<blockquote>
<P>The <A HREF="http://www.genenetwork.org/mouseCross.html#LXS" target="_empty" class="fs14">LXS</A> genetic reference panel of recombinant inbred strains consists of just over 77 strains. All of these strains have been inbred for more than 23 generations (F23). All strains have been genotyped at 13,377 SNPs. Thanks to the efforts of <A HREF="http://www.gnf.org/technology/organismal/mamm-genetics-haplotype-mutation-mapping.htm" target="_empty" class="fs14">Dr. Timothy Wiltshire</A> at the Genome Institute of the Novartis Research Foundation, the two parental strains have been genotyped at 156,551 SNPs. These genotypes are incorporated in the GeneNetwork <A HREF="http://www.genenetwork.org/webqtl/snpBrowser.py" target="_empty" class="fs14">SNP Browser</A>.


<P>Strains are currently available from Drs. Beth Bennett and Tom Johnson at the <A HREF="http://ibgwww.colorado.edu/" target="_empty" class="fs14">Institute of Behavioral Genetics</A> (IBG) in Boulder Colorado. </P>

</Blockquote>

</Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;About the animals and tissue used to generate this set of data:</P>

<Blockquote><P>All animals were raised at the IBG by Bennett and colleagues in an SPF facility. No cases were MHV positive. All animals were treated and brains dissected by Chris Downing and colleagues at IBG.  Dissections were according to protocols routinely used by the Miles' laboratory (Kerns et al., J. Neurosci. 2005).  Prefrontal cortex tissue was isolated by microdissection using a wedge-shaped slice taken from a 4 mm thick brain slice extending rostrally from the optic chiasm.  The wedge was centered on the inter-hemispheric fissure and extending 2 mm laterally on each side and ventrally to just above the corpus callosum. This tissue and all other brain regions were dissected in less than 5 minutes per mouse and were immediated frozen in liquid nitrogen followed by storage at -80 oC prior to RNA isolation.

<P>A pool of dissected tissue from 3-4 adults of the same strain, sex, age and treatment group (saline or ethanol) was used to generate RNA samples. The great majority (75%) of animals were sacrificed between 9:30 AM and 11:30 AM. All animals were sacrificed between 9 AM and 5 PM during the light phase. All RNA samples were extracted at VCU by Paul Vorster between 1/06 and 2/06.  Strains were randomized in terms of order of RNA isolation but paired saline and ethanol samples from the same strain were always processed at the same time to decrease technical causes for differences in gene expression between saline and ethanol treated animals.

<P>All animals used in this study were between 53 and 90 days of age (average of 72 days; see Table 1 below).
</Blockquote>

<Blockquote>
<P><B>Sample Processing:</B> Samples were processed by Paul Vorster at VCU between 2/06 and 4/06. In brief, RNA purity was evaluated using the 260/280 nm absorbance ratio with all values between 1.9 and 2.1. Total RNA integrity was assessed using the BioRad Experion RNA analyzer and used to verify equal molar ratios of 18S and 28S ribosomal RNA. Standard Affymetrix reagents and protocols were used for generation of cDNA and biotinylated cRNA from total RNA samples.  Integrity of cRNA was checked by Experion analysis prior to microarray hybridizations.  All probes exceeded a maximum size of 3000 nt for the upper border of the cRNA size distribution.

<P><B>Replication and Sample Balance:</B> At present, all strains are represented by a total of 2 microrarrays, one each for saline- and ethanol-treated animals.


<P><B>Experimental Design and Batch Structure:</B> CHANGE THIS This data set consists arrays processed in 13 groups over a five month period (July 2006 to Dec 2006). Most groups consisted of 12 samples. All arrays in this data set were processed using a single protocol by a single operator, Feng Yiao. Processing was supervised directly by Dr. Lu Lu. All samples were scanned on a single Illumina Beadstation housed in the Hamilton Eye Institute between July 28 and Dec 21, 2006. Details on sample assignment to slides and batches is provide in the table below.


</Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;Data Table 1:</P>

<Blockquote>

<Blockquote>
CHANGE THIS This table lists all arrays by order of strain (<B>index</B>) and includes data on tube ID, strain, age, sex, F generation number, number of animals in each sample pool (<B>pool size</B>), slide ID, slide position (A through F), scan date, and scan batch.
</Blockquote>


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<tr bgcolor="royalblue">
<td><font color=#FFFFFF>index</font></td>
<td><font color=#FFFFFF>tube ID</font></td>
<td><font color=#FFFFFF>strain</td>
<td><font color=#FFFFFF>age</font></td>
<td><font color=#FFFFFF>sex</font></td>
<td><font color=#FFFFFF>gener-<BR>ation</td>
<td><font color=#FFFFFF>pool <BR>size</td>
<td><font color=#FFFFFF>slide <BR>ID</font></td>
<td><font color=#FFFFFF>slide <BR>position</font></td>
<td><font color=#FFFFFF>batch <BR>by slide</font></td>
<td><font color=#FFFFFF>scan <BR>batch</td>
</tr>


<!-- Rob Removed the F1s from this table  -->


<tr bgcolor="#eeeeee"><td>1</td><td>R2851H2</td><td>ILS</td><td>77</td><td>F</td><td>>100</td><td>4</td><td>1523516003</td><td>B</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>2</td><td>R0595H2</td><td>ILS</td><td>71</td><td>F</td><td>>100</td><td>2</td><td>1523516030</td><td>B</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>3</td><td>R2874H2</td><td>ILS</td><td>78</td><td>M</td><td>>100</td><td>4</td><td>1523516011</td><td>B</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>4</td><td>R0585H2</td><td>ILS</td><td>65</td><td>M</td><td>>100</td><td>2</td><td>1523516028</td><td>A</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>6</td><td>R2857H2</td><td>ISS</td><td>75</td><td>F</td><td>>100</td><td>4</td><td>1523516011</td><td>A</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>7</td><td>R0589H2</td><td>ISS</td><td>73</td><td>F</td><td>>100</td><td>2</td><td>1523516028</td><td>B</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>8</td><td>R2955H2</td><td>ISS</td><td>53</td><td>M</td><td>>100</td><td>3</td><td>1523516003</td><td>A</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>9</td><td>R0578H2</td><td>ISS</td><td>67</td><td>M</td><td>>100</td><td>2</td><td>1523516030</td><td>A</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>11</td><td>R2013H2</td><td>LXS10</td><td>84</td><td>F</td><td>27</td><td>2</td><td>1562224049</td><td>E</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>12</td><td>R1993H2</td><td>LXS10</td><td>78</td><td>M</td><td>27</td><td>2</td><td>1562224036</td><td>E</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>13</td><td>R1997H2</td><td>LXS100</td><td>70</td><td>F</td><td>25</td><td>2</td><td>1562224049</td><td>A</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>14</td><td>R1983H2</td><td>LXS100</td><td>77</td><td>M</td><td>25</td><td>2</td><td>1562224047</td><td>F</td><td>16</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>15</td><td>R2083H2</td><td>LXS101</td><td>85</td><td>F</td><td>28</td><td>2</td><td>1562224050</td><td>B</td><td>19</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>16</td><td>R2084H2</td><td>LXS101</td><td>85</td><td>M</td><td>28</td><td>2</td><td>1562224050</td><td>C</td><td>19</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>17</td><td>R2186H2</td><td>LXS102</td><td>73</td><td>F</td><td>29</td><td>2</td><td>1523516001</td><td>C</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>18</td><td>R2187H2</td><td>LXS102</td><td>73</td><td>M</td><td>29</td><td>2</td><td>1562224053</td><td>C</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>19</td><td>R2809H2</td><td>LXS103</td><td>72</td><td>F</td><td>26</td><td>2</td><td>1562224034</td><td>C</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>20</td><td>R2854H1</td><td>LXS103</td><td>69</td><td>M</td><td>26</td><td>2</td><td>1523516006</td><td>D</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>21</td><td>R2735H2</td><td>LXS107</td><td>78</td><td>F</td><td>26</td><td>2</td><td>1523516028</td><td>D</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>22</td><td>R2738H2</td><td>LXS107</td><td>78</td><td>M</td><td>26</td><td>2</td><td>1562224054</td><td>C</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>23</td><td>R2840H2</td><td>LXS110</td><td>67</td><td>F</td><td>25</td><td>2</td><td>1562224037</td><td>D</td><td>24</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>24</td><td>R2157H2</td><td>LXS110</td><td>75</td><td>M</td><td>27</td><td>2</td><td>1523516033</td><td>E</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>25</td><td>R2172H2</td><td>LXS112</td><td>72</td><td>F</td><td>27</td><td>2</td><td>1523516001</td><td>B</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>26</td><td>R2841H2</td><td>LXS112</td><td>84</td><td>M</td><td>27</td><td>2</td><td>1562224042</td><td>D</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>27</td><td>R2188H2</td><td>LXS114</td><td>72</td><td>F</td><td>28</td><td>2</td><td>1562224053</td><td>D</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>28</td><td>R2204H2</td><td>LXS114</td><td>72</td><td>M</td><td>28</td><td>2</td><td>1523516001</td><td>D</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>29</td><td>R2168H2</td><td>LXS115</td><td>80</td><td>F</td><td>27</td><td>2</td><td>1523516001</td><td>A</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>30</td><td>R2085H2</td><td>LXS115</td><td>71</td><td>M</td><td>27</td><td>2</td><td>1562224050</td><td>D</td><td>19</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>31</td><td>R2811H2</td><td>LXS117</td><td>72</td><td>F</td><td>28</td><td>2</td><td>1562224034</td><td>D</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>32</td><td>R2212H2</td><td>LXS117</td><td>74</td><td>M</td><td>27</td><td>2</td><td>1523516001</td><td>E</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>33</td><td>R1992H2</td><td>LXS122</td><td>86</td><td>F</td><td>26</td><td>2</td><td>1562224036</td><td>D</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>34</td><td>R2219H2</td><td>LXS122</td><td>72</td><td>M</td><td>26</td><td>2</td><td>1562224054</td><td>A</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>35</td><td>R2876H2</td><td>LXS123</td><td>87</td><td>F</td><td>28</td><td>2</td><td>1562224038</td><td>C</td><td>25</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>36</td><td>R2832H1</td><td>LXS123</td><td>77</td><td>M</td><td>27</td><td>2</td><td>1523516011</td><td>E</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>37</td><td>R2872H1</td><td>LXS124</td><td>83</td><td>F</td><td>28</td><td>2</td><td>1523516006</td><td>E</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>38</td><td>R2871H2</td><td>LXS124</td><td>85</td><td>M</td><td>28</td><td>2</td><td>1562224042</td><td>F</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>39</td><td>R1909H2</td><td>LXS13</td><td>79</td><td>F</td><td>25</td><td>2</td><td>1523516024</td><td>D</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>40</td><td>R1901H2</td><td>LXS13</td><td>81</td><td>M</td><td>26</td><td>2</td><td>1562224041</td><td>D</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>41</td><td>R2023H2</td><td>LXS14</td><td>86</td><td>F</td><td>25</td><td>2</td><td>1562224050</td><td>A</td><td>19</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>42</td><td>R1612H2</td><td>LXS14</td><td>85</td><td>M</td><td>26</td><td>2</td><td>1523516015</td><td>A</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>43</td><td>R1936H2</td><td>LXS16</td><td>81</td><td>F</td><td>25</td><td>2</td><td>1523516024</td><td>E</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>44</td><td>R1912H2</td><td>LXS16</td><td>81</td><td>M</td><td>25</td><td>2</td><td>1523516033</td><td>B</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>45</td><td>R1961H2</td><td>LXS19</td><td>66</td><td>F</td><td>26</td><td>2</td><td>1523516029</td><td>F</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>46</td><td>R1904H2</td><td>LXS19</td><td>66</td><td>M</td><td>26</td><td>2</td><td>1523516024</td><td>B</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>47</td><td>R1883H2</td><td>LXS2</td><td>71</td><td>F</td><td>27</td><td>2</td><td>1523516009</td><td>F</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>48</td><td>R2753H2</td><td>LXS2</td><td>74</td><td>M</td><td>28</td><td>2</td><td>1523516030</td><td>E</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>49</td><td>R1729H2</td><td>LXS22</td><td>72</td><td>F</td><td>27</td><td>2</td><td>1523516032</td><td>E</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>50</td><td>R2743H2</td><td>LXS22</td><td>79</td><td>M</td><td>27</td><td>2</td><td>1562224054</td><td>D</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>51</td><td>R1966H2</td><td>LXS23</td><td>73</td><td>F</td><td>26</td><td>2</td><td>1562224032</td><td>D</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>52</td><td>R1971H2</td><td>LXS23</td><td>73</td><td>M</td><td>26</td><td>2</td><td>1562224047</td><td>A</td><td>16</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>53</td><td>R2795H2</td><td>LXS24</td><td>74</td><td>F</td><td>26</td><td>2</td><td>1523516003</td><td>C</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>54</td><td>R1755H2</td><td>LXS24</td><td>56</td><td>M</td><td>25</td><td>2</td><td>1523516007</td><td>A</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>55</td><td>R1986H2</td><td>LXS25</td><td>75</td><td>F</td><td>27</td><td>2</td><td>1562224036</td><td>A</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>56</td><td>R2006H2</td><td>LXS25</td><td>75</td><td>M</td><td>27</td><td>2</td><td>1562224049</td><td>C</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>57</td><td>R2014H2</td><td>LXS26</td><td>82</td><td>F</td><td>25</td><td>2</td><td>1562224049</td><td>F</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>58</td><td>R2009H2</td><td>LXS26</td><td>82</td><td>M</td><td>26</td><td>2</td><td>1562224049</td><td>D</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>59</td><td>R2824H1</td><td>LXS28</td><td>67</td><td>F</td><td>28</td><td>2</td><td>1562224042</td><td>A</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>60</td><td>R1753H1</td><td>LXS28</td><td>72</td><td>M</td><td>27</td><td>2</td><td>1562224041</td><td>F</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>61</td><td>R2765H2</td><td>LXS3</td><td>63</td><td>F</td><td>27</td><td>2</td><td>1523516005</td><td>B</td><td>5</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>62</td><td>R1898H2</td><td>LXS3</td><td>81</td><td>M</td><td>26</td><td>2</td><td>1562224041</td><td>C</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>63</td><td>R2764H2</td><td>LXS31</td><td>73</td><td>F</td><td>29</td><td>2</td><td>1562224060</td><td>C</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>64</td><td>R1908H2</td><td>LXS31</td><td>80</td><td>M</td><td>28</td><td>2</td><td>1523516024</td><td>C</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>65</td><td>R2758H2</td><td>LXS32</td><td>70</td><td>F</td><td>26</td><td>2</td><td>1523516030</td><td>F</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>66</td><td>R1743H1</td><td>LXS32</td><td>67</td><td>M</td><td>25</td><td>2</td><td>1562224041</td><td>E</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>67</td><td>R2794H2</td><td>LXS34</td><td>75</td><td>F</td><td>27</td><td>2</td><td>1562224034</td><td>B</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>68</td><td>R2870H2</td><td>LXS34</td><td>78</td><td>F</td><td>27</td><td>2</td><td>1562224029</td><td>C</td><td>28</td><td>13</td></tr>
<tr bgcolor="#eeeeee"><td>69</td><td>R2746H2</td><td>LXS35</td><td>80</td><td>F</td><td>22</td><td>2</td><td>1523516030</td><td>C</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>70</td><td>R2747H2</td><td>LXS35</td><td>81</td><td>M</td><td>22</td><td>2</td><td>1562224060</td><td>A</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>71</td><td>R1968H2</td><td>LXS36</td><td>76</td><td>F</td><td>25</td><td>2</td><td>1562224032</td><td>E</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>72</td><td>R1640H2</td><td>LXS36</td><td>76</td><td>M</td><td>25</td><td>2</td><td>1523516032</td><td>C</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>73</td><td>R2835H1</td><td>LXS38</td><td>67</td><td>F</td><td>27</td><td>2</td><td>1523516011</td><td>F</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>74</td><td>R2842H2</td><td>LXS38</td><td>67</td><td>M</td><td>27</td><td>2</td><td>1562224042</td><td>E</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>75</td><td>R2210H2</td><td>LXS39</td><td>81</td><td>F</td><td>28</td><td>2</td><td>1562224053</td><td>E</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>76</td><td>R2736H2</td><td>LXS39</td><td>75</td><td>M</td><td>27</td><td>2</td><td>1523516028</td><td>E</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>77</td><td>R1978H2</td><td>LXS41</td><td>78</td><td>F</td><td>26</td><td>2</td><td>1562224047</td><td>C</td><td>16</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>78</td><td>R1783H2</td><td>LXS41</td><td>56</td><td>M</td><td>26</td><td>2</td><td>1523516007</td><td>D</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>79</td><td>R2822H2</td><td>LXS42</td><td>66</td><td>F</td><td>27</td><td>2</td><td>1562224034</td><td>F</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>80</td><td>R2769H2</td><td>LXS42</td><td>70</td><td>M</td><td>27</td><td>2</td><td>1562224042</td><td>B</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>81</td><td>R1974H2</td><td>LXS43</td><td>84</td><td>F</td><td>26</td><td>2</td><td>1562224047</td><td>B</td><td>16</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>82</td><td>R1733H2</td><td>LXS43</td><td>72</td><td>M</td><td>26</td><td>2</td><td>1523516015</td><td>C</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>83</td><td>R1756H2</td><td>LXS46</td><td>56</td><td>F</td><td>25</td><td>2</td><td>1523516007</td><td>B</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>84</td><td>R1727H2</td><td>LXS46</td><td>70</td><td>M</td><td>25</td><td>2</td><td>1523516032</td><td>D</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>85</td><td>R1970H2</td><td>LXS48</td><td>72</td><td>F</td><td>27</td><td>2</td><td>1562224032</td><td>F</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>86</td><td>R1981H2</td><td>LXS48</td><td>72</td><td>M</td><td>27</td><td>2</td><td>1562224042</td><td>C</td><td>27</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>87</td><td>R1957H2</td><td>LXS49</td><td>72</td><td>F</td><td>25</td><td>2</td><td>1523516029</td><td>C</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>88</td><td>R2259H2</td><td>LXS49</td><td>72</td><td>M</td><td>25</td><td>2</td><td>1523516028</td><td>C</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>89</td><td>R2836H1</td><td>LXS5</td><td>68</td><td>F</td><td>28</td><td>2</td><td>1523516006</td><td>A</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>90</td><td>R2213H2</td><td>LXS5</td><td>80</td><td>M</td><td>27</td><td>2</td><td>1562224053</td><td>F</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>91</td><td>R2791H2</td><td>LXS50</td><td>68</td><td>F</td><td>27</td><td>2</td><td>1562224034</td><td>A</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>92</td><td>R1789H2</td><td>LXS50</td><td>57</td><td>M</td><td>26</td><td>2</td><td>1523516033</td><td>A</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>93</td><td>R1740H2</td><td>LXS51</td><td>68</td><td>F</td><td>27</td><td>2</td><td>1523516032</td><td>F</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>94</td><td>R1734H2</td><td>LXS51</td><td>68</td><td>M</td><td>26</td><td>2</td><td>1523516015</td><td>D</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>95</td><td>R2786H2</td><td>LXS52</td><td>61</td><td>F</td><td>27</td><td>2</td><td>1562224060</td><td>D</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>96</td><td>R2768H2</td><td>LXS52</td><td>61</td><td>M</td><td>27</td><td>2</td><td>1523516005</td><td>C</td><td>5</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>97</td><td>R2154H2</td><td>LXS54</td><td>70</td><td>F</td><td>27</td><td>2</td><td>1523516033</td><td>D</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>98</td><td>R2155H2</td><td>LXS54</td><td>70</td><td>M</td><td>27</td><td>2</td><td>1562224053</td><td>A</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>99</td><td>R1821H2</td><td>LXS55</td><td>77</td><td>F</td><td>25</td><td>2</td><td>1523516009</td><td>C</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>100</td><td>R1951H2</td><td>LXS55</td><td>74</td><td>M</td><td>26</td><td>2</td><td>1523516024</td><td>F</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>101</td><td>R2789H2</td><td>LXS56</td><td>71</td><td>F</td><td>25</td><td>2</td><td>1523516005</td><td>F</td><td>5</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>102</td><td>R2788H2</td><td>LXS56</td><td>71</td><td>M</td><td>25</td><td>2</td><td>1562224060</td><td>F</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>103</td><td>R2787H2</td><td>LXS59</td><td>66</td><td>F</td><td>29</td><td>2</td><td>1562224060</td><td>E</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>104</td><td>R2785H2</td><td>LXS59</td><td>62</td><td>M</td><td>29</td><td>2</td><td>1523516005</td><td>E</td><td>5</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>105</td><td>R1791H2</td><td>LXS60</td><td>58</td><td>F</td><td>27</td><td>2</td><td>1562224038</td><td>D</td><td>25</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>106</td><td>R1792H2</td><td>LXS60</td><td>64</td><td>M</td><td>27</td><td>2</td><td>1523516007</td><td>E</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>107</td><td>R1796H2</td><td>LXS62</td><td>58</td><td>F</td><td>27</td><td>2</td><td>1523516007</td><td>F</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>108</td><td>R1797H2</td><td>LXS62</td><td>58</td><td>M</td><td>27</td><td>2</td><td>1562224038</td><td>E</td><td>25</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>109</td><td>R2220H2</td><td>LXS64</td><td>71</td><td>F</td><td>28</td><td>2</td><td>1523516001</td><td>F</td><td>9</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>110</td><td>R2221H2</td><td>LXS64</td><td>71</td><td>M</td><td>28</td><td>2</td><td>1562224054</td><td>B</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>111</td><td>R1989H2</td><td>LXS66</td><td>73</td><td>F</td><td>26</td><td>2</td><td>1562224036</td><td>B</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>112</td><td>R1843H2</td><td>LXS66</td><td>78</td><td>M</td><td>27</td><td>2</td><td>1523516009</td><td>E</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>113</td><td>R2820H2</td><td>LXS68</td><td>67</td><td>F</td><td>29</td><td>2</td><td>1523516003</td><td>E</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>114</td><td>R2819H2</td><td>LXS68</td><td>67</td><td>M</td><td>29</td><td>2</td><td>1562224034</td><td>E</td><td>23</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>115</td><td>R1963H2</td><td>LXS7</td><td>78</td><td>F</td><td>28</td><td>2</td><td>1562224032</td><td>B</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>116</td><td>R1964H2</td><td>LXS7</td><td>78</td><td>M</td><td>28</td><td>2</td><td>1562224032</td><td>C</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>117</td><td>R2166H2</td><td>LXS70</td><td>72</td><td>F</td><td>27</td><td>2</td><td>1523516033</td><td>F</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>118</td><td>R2745H2</td><td>LXS70</td><td>71</td><td>M</td><td>28</td><td>2</td><td>1562224054</td><td>F</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>119</td><td>R2848H2</td><td>LXS72</td><td>72</td><td>F</td><td>27</td><td>2</td><td>1562224038</td><td>A</td><td>25</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>120</td><td>R1902H2</td><td>LXS72</td><td>66</td><td>M</td><td>27</td><td>2</td><td>1523516024</td><td>A</td><td>13</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>121</td><td>R2750H2</td><td>LXS73</td><td>81</td><td>F</td><td>25</td><td>2</td><td>1523516030</td><td>D</td><td>4</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>122</td><td>R1835H2</td><td>LXS73</td><td>90</td><td>M</td><td>24</td><td>2</td><td>1523516009</td><td>D</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>123</td><td>R1979H2</td><td>LXS75</td><td>59</td><td>F</td><td>27</td><td>2</td><td>1562224047</td><td>D</td><td>16</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>124</td><td>R2826H2</td><td>LXS75</td><td>72</td><td>M</td><td>27</td><td>2</td><td>1523516003</td><td>F</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>125</td><td>R2142H2</td><td>LXS76</td><td>77</td><td>F</td><td>26</td><td>2</td><td>1562224050</td><td>E</td><td>19</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>126</td><td>R1884H2</td><td>LXS76</td><td>85</td><td>M</td><td>26</td><td>2</td><td>1562224041</td><td>A</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>127</td><td>R1959H2</td><td>LXS78</td><td>69</td><td>F</td><td>26</td><td>2</td><td>1523516029</td><td>E</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>128</td><td>R1958H2</td><td>LXS78</td><td>69</td><td>M</td><td>26</td><td>2</td><td>1523516029</td><td>D</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>129</td><td>R2845H1</td><td>LXS8</td><td>70</td><td>F</td><td>28</td><td>2</td><td>1523516006</td><td>C</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>130</td><td>R2156H2</td><td>LXS8</td><td>76</td><td>M</td><td>27</td><td>2</td><td>1562224053</td><td>B</td><td>20</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>131</td><td>R1955H2</td><td>LXS80</td><td>71</td><td>F</td><td>25</td><td>2</td><td>1523516029</td><td>A</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>132</td><td>R1956H2</td><td>LXS80</td><td>71</td><td>M</td><td>25</td><td>2</td><td>1523516029</td><td>B</td><td>14</td><td>7</td></tr>
<tr bgcolor="#eeeeee"><td>133</td><td>R2830H1</td><td>LXS84</td><td>66</td><td>F</td><td>26</td><td>2</td><td>1523516011</td><td>D</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>134</td><td>R2829H2</td><td>LXS84</td><td>66</td><td>M</td><td>26</td><td>2</td><td>1562224037</td><td>A</td><td>24</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>135</td><td>R2839H2</td><td>LXS86</td><td>68</td><td>F</td><td>27</td><td>2</td><td>1562224037</td><td>C</td><td>24</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>136</td><td>R2838H1</td><td>LXS86</td><td>68</td><td>M</td><td>27</td><td>2</td><td>1523516006</td><td>B</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>137</td><td>R2882H1</td><td>LXS87</td><td>66</td><td>F</td><td>27</td><td>2</td><td>1523516006</td><td>F</td><td>6</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>138</td><td>R2744H2</td><td>LXS87</td><td>71</td><td>M</td><td>26</td><td>2</td><td>1562224054</td><td>E</td><td>21</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>139</td><td>R2831H2</td><td>LXS88</td><td>69</td><td>F</td><td>27</td><td>2</td><td>1562224037</td><td>B</td><td>24</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>140</td><td>R2762H2</td><td>LXS88</td><td>71</td><td>M</td><td>27</td><td>2</td><td>1523516005</td><td>A</td><td>5</td><td>3</td></tr>
<tr bgcolor="#eeeeee"><td>141</td><td>R2828H1</td><td>LXS89</td><td>75</td><td>F</td><td>26</td><td>2</td><td>1523516011</td><td>C</td><td>2</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>142</td><td>R1962H2</td><td>LXS89</td><td>73</td><td>M</td><td>25</td><td>2</td><td>1562224032</td><td>A</td><td>15</td><td>8</td></tr>
<tr bgcolor="#eeeeee"><td>143</td><td>R1746H2</td><td>LXS9</td><td>66</td><td>F</td><td>26</td><td>2</td><td>1523516015</td><td>F</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>144</td><td>R2801H2</td><td>LXS9</td><td>68</td><td>M</td><td>27</td><td>2</td><td>1523516003</td><td>D</td><td>1</td><td>1</td></tr>
<tr bgcolor="#eeeeee"><td>145</td><td>R1812H2</td><td>LXS90</td><td>61</td><td>F</td><td>25</td><td>2</td><td>1562224038</td><td>F</td><td>25</td><td>11</td></tr>
<tr bgcolor="#eeeeee"><td>146</td><td>R1813H2</td><td>LXS90</td><td>61</td><td>M</td><td>25</td><td>2</td><td>1523516009</td><td>A</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>147</td><td>R1736H2</td><td>LXS92</td><td>66</td><td>F</td><td>23</td><td>2</td><td>1523516015</td><td>E</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>148</td><td>R1609H2</td><td>LXS92</td><td>87</td><td>M</td><td>23</td><td>2</td><td>1523516032</td><td>A</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>149</td><td>R1624H2</td><td>LXS93</td><td>74</td><td>F</td><td>26</td><td>2</td><td>1523516032</td><td>B</td><td>7</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>150</td><td>R1815H2</td><td>LXS93</td><td>61</td><td>M</td><td>26</td><td>2</td><td>1523516009</td><td>B</td><td>12</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>151</td><td>R1991H2</td><td>LXS94</td><td>70</td><td>F</td><td>25</td><td>2</td><td>1562224036</td><td>C</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>152</td><td>R2002H2</td><td>LXS94</td><td>70</td><td>M</td><td>25</td><td>2</td><td>1562224049</td><td>B</td><td>18</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>153</td><td>R1996H2</td><td>LXS96</td><td>75</td><td>F</td><td>23</td><td>2</td><td>1562224036</td><td>F</td><td>17</td><td>9</td></tr>
<tr bgcolor="#eeeeee"><td>154</td><td>R1772H2</td><td>LXS96</td><td>63</td><td>M</td><td>23</td><td>2</td><td>1523516007</td><td>C</td><td>11</td><td>6</td></tr>
<tr bgcolor="#eeeeee"><td>155</td><td>R2759H2</td><td>LXS97</td><td>73</td><td>F</td><td>26</td><td>2</td><td>1562224060</td><td>B</td><td>22</td><td>10</td></tr>
<tr bgcolor="#eeeeee"><td>156</td><td>R2739H2</td><td>LXS97</td><td>79</td><td>M</td><td>26</td><td>2</td><td>1523516028</td><td>F</td><td>3</td><td>2</td></tr>
<tr bgcolor="#eeeeee"><td>157</td><td>R2149H2</td><td>LXS98</td><td>78</td><td>F</td><td>26</td><td>2</td><td>1523516033</td><td>C</td><td>8</td><td>4</td></tr>
<tr bgcolor="#eeeeee"><td>158</td><td>R1888H2</td><td>LXS98</td><td>76</td><td>M</td><td>26</td><td>2</td><td>1562224041</td><td>B</td><td>26</td><td>12</td></tr>
<tr bgcolor="#eeeeee"><td>159</td><td>R1644H2</td><td>LXS99</td><td>79</td><td>F</td><td>26</td><td>2</td><td>1523516015</td><td>B</td><td>10</td><td>5</td></tr>
<tr bgcolor="#eeeeee"><td>160</td><td>R2145H2</td><td>LXS99</td><td>77</td><td>M</td><td>27</td><td>2</td><td>1562224050</td><td>F</td><td>19</td><td>9</td></tr>
</table>
</td>
</tr>
</table>
</Blockquote>
</Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;Downloading all data:</P>
<Blockquote>
<P>All data links (right-most column above) will be made active as soon as the global analysis of these data has been accepted for publication. Please see text on <A HREF="http://www.genenetwork.org/dataSharing.html" target="_empty" class="fs14">Data Sharing Policies</A>, and <A HREF="http://www.genenetwork.org/conditionsofUse.html" target="_empty" class="fs14">Conditions and Limitations</A>, and <A HREF="http://www.genenetwork.org/statusandContact.html" target="_empty" class="fs14">Contacts</A>. Following publication, download a summary text file or Excel file of data. Please contact <A class="fs14" HREF="mailto:lulu@nb.utmem.edu">Dr. Lu Lu</A> if you have any questions on the use of these open data. </P>
</Blockquote>




<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;About the array platform:</P>
<Blockquote>
<P><B>PLEASE ADD TEXT REGARDING AFFYMETRIX M430A version 2
</Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;About data processing:</P>

<Blockquote>
<P>ADD TEXT


</BLOCKQUOTE>

</Blockquote>


<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;Data source acknowledgment:</P>
<Blockquote><P>Data were generated with funds to Lu Lu, Beth Bennett, Mike Miles, Melloni Cook from INIA.

<LI>Lu Lu, M.D.  <!--Tissue acquisition, RNA processing, experimental design-->
<BR>Grant Support: PLEASE ADD
</UL>



</P></Blockquote>

<P class="subtitle">&nbsp;&nbsp;&nbsp;&nbsp;About this text file:</P>
<Blockquote><P>
Data uploaded by Hongqiang Li, Oct 30, 2006. This text file originally generated by LL and RWW on November 29, 2006. Updated by LL, Dec 1, 2006. Updated March 25, April 25, 2007 by RWW. Updated by RWW, Nov 2007 (removed references to Illumina).


<P>


</P></Blockquote>



<P></P>

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