UCLA CAST/Ei X C57BL/6J reciprocal F2 Intercross Brain (2005) mlratio

SUMMARY: One of a set of four large expression data set (brain, peritoneal fat, liver, and quadriceps muscle) by Alfons Jake Lusis and colleagues (UCLA) generated using two large reciprocal F2 intercrosses between CAST/EiJ and C57BL/6J. The data are given as mean log ratios. These values are essentially expression offsets for each individual with respect to the group mean.

Breeding and tissue dissection was performed at UCLA by AJL and colleagues.

The brain in this case consists of the olfactory bulbs, the whole forebrain and midbrain, the cerebellum, and hindbrain. Data are provided for 401 animals, roughly the same number of reciprocal BCF2 and CBF2 progeny.

RNA extraction, expression profiling, genotyping, and initial data transformation and normalization were carried out by Eric Schadt and colleagues at Rosetta/Merck.

A custom Agilent array consisting of 23,272 60-mer probes was used in this study. The same array was also used in a study of the same four tissues in a large BHF2 intercross (also included in GeneNetwork).

Data entered by Evan Williams (July 24, 2008).

These unapproved notes by RWW, July 25, 2008.