<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML><HEAD><TITLE>UCLA BXD and BXH Cartilage</TITLE> <META http-equiv=Content-Type content="text/html; charset=iso-8859-1"> <LINK REL="stylesheet" TYPE="text/css" HREF='/css/general.css'> <LINK REL="stylesheet" TYPE="text/css" HREF='/css/menu.css'> <SCRIPT SRC="/javascript/webqtl.js"></SCRIPT> <SCRIPT SRC="/javascript/dhtml.js"></SCRIPT> </HEAD> <BODY bottommargin="2" leftmargin="2" rightmargin="2" topmargin="2" text=#000000 bgColor=#ffffff > <TABLE cellSpacing=5 cellPadding=4 width="100%" border=0> <TBODY> <TR> <script language="JavaScript" src="/javascript/header.js"></script> </TR> <TR> <TD bgColor=#eeeeee class="solidBorder"> <Table width= "100%" cellSpacing=0 cellPadding=5> <TR> <!-- split from Here --> <!-- Body Start from Here --> <P class="title">UCLA BXD and BXH Cartilage <A HREF="/webqtl/main.py?FormID=editHtml"> <img src="/images/modify.gif" alt="modify this page" border= 0 valign="middle"></A><BR><BR>Accession number: <A HREF="/webqtl/main.py?FormID=sharinginfo&GN_AccessionId=205">GN205</A></P> <P>PRELIMINARY SUMMARY (private data): One of a set of two expression data sets by Alfons Jake Lusis and colleagues (UCLA) generated using two sets of Recombinant Inbred strains. <P>General information and background for BXD and BXH UCLA Cartilage data sets: <OL> <LI>BXD UCLA data sets <LI>BXH UCLA data sets </OL> <P>All mice were maintained on a 12 h light–12 h dark cycle and fed ad libitum. <P>Mice were killed at XX weeks, and tissues, including the cartilage were immediately collected and flash-frozen in liquid nitrogen. <P>All procedures of housing and treatment of animals were performed in accordance with Institutional Animal Care and Use Committee regulations (UCLA). <P><B>Array design and preparation of labeled cDNA and hybridizations to microarrays for the mouse tissue samples.</B> RNA preparation and array hybridizations were performed at UCLA using Illumina Mouse 6 version 1.1 arrays. <P>Mouse cartilage was homogenized, and total RNA was extracted using Trizol (Invitrogen) according to manufacturer's protocol. ADD MORE. <P>Arrays were quantified using Illumina Rank Invariant protocol and normalized to a mean of 8 units and a SEM of all probes to 2.0 units (a modified Z score). <P>Data entered by Evan Williams (July 24, 2008). <P>These unapproved notes by RWW, Sept 4, 2008. </TR></TABLE> </TD> </TR> <TR> <TD align=center bgColor=#ddddff class="solidBorder"> <!--Start of footer--> <TABLE width="90%"> <script language='JavaScript' src='/javascript/footer.js'></script> <TR> <TD colspan=3 class="fs12"> <UL> </UL> </TD> </TR> </TABLE> <!--End of footer--> </TD> </TR> </TABLE> <!-- /Footer --> <!-- menu script itself. you should not modify this file --> <script language="JavaScript" src="/javascript/menu_new.js"></script> <!-- items structure. menu hierarchy and links are stored there --> <script language="JavaScript" src="/javascript/menu_items.js"></script> <!-- files with geometry and styles structures --> <script language="JavaScript" src="/javascript/menu_tpl.js"></script> <script language="JavaScript"> <!--// // Note where menu initialization block is located in HTML document. // Don't try to position menu locating menu initialization block in // some table cell or other HTML element. Always put it before </body> // each menu gets two parameters (see demo files) // 1. items structure // 2. geometry structure new menu (MENU_ITEMS, MENU_POS); // make sure files containing definitions for these variables are linked to the document // if you got some javascript error like "MENU_POS is not defined", then you've made syntax // error in menu_tpl.js file or that file isn't linked properly. // also take a look at stylesheets loaded in header in order to set styles //--> </script> </BODY> </HTML>