UCLA BXD and BXH Cartilage modify this page

Accession number: GN204

PRELIMINARY SUMMARY (private data): One of a set of two expression data sets by Alfons Jake Lusis and colleagues (UCLA) generated using two sets of Recombinant Inbred strains.

General information and background for BXD and BXH UCLA Cartilage data sets:

  1. BXD UCLA data sets
  2. BXH UCLA data sets

All mice were maintained on a 12 h light–12 h dark cycle and fed ad libitum.

Mice were killed at XX weeks, and tissues, including the cartilage were immediately collected and flash-frozen in liquid nitrogen.

All procedures of housing and treatment of animals were performed in accordance with Institutional Animal Care and Use Committee regulations (UCLA).

Array design and preparation of labeled cDNA and hybridizations to microarrays for the mouse tissue samples. RNA preparation and array hybridizations were performed at UCLA using Illumina Mouse 6 version 1.1 arrays.

Mouse cartilage was homogenized, and total RNA was extracted using Trizol (Invitrogen) according to manufacturer's protocol. ADD MORE.

Arrays were quantified using Illumina Rank Invariant protocol and normalized to a mean of 8 units and a SEM of all probes to 2.0 units (a modified Z score).

Data entered by Evan Williams (July 24, 2008).

These unapproved notes by RWW, Sept 4, 2008.