U74Av2 Mas5 Original Database (December/03 Freeze)

Accession number: GN15

    About the mice used to map microarray data:

Original Affmetrix values without logarithm or standardization. The set of animals used for mapping (a mapping panel) consists of groups of genetically uniform mice of the BXD type. The parental strains are C57BL/6J (B6 or B) and DBA/2J (D2 or D). The first generation hybrid is labeled F1. The F1 hybrids were made by crossing B6 females to D2 males. All other lines are recombinant inbred strains derived from C57BL/6J and DBA/2J crosses. BXD2 through BXD32 were produced by Dr. Benjamin Taylor starting in the late 1970s. BXD33 through BXD42 were also produced by Dr. Taylor, but they were generated in the 1990s. Lines BXD67 and BXD68 are two partially inbred advanced recombinant strains (F8 and F9) that are part of a large set of BXD-Advanced strains being produced by Drs. Robert Williams, Lu Lu, Guomin Zhou, Lee Silver, and Jeremy Peirce. There will eventually be ~45 of these strains. For additional background on recombinant inbred strains, please see http://www.nervenet.org/papers/bxn.html.

The table below lists the arrays by strain, sex, and age. Each array was hybridized to a pool of mRNA from 3 mice.

    How to download these data:

All standard Affymetrix file types (DAT, CEL, RPT, CHP, TXT) can be downloaded for this data set by selecting the strain names in the table above and then selecting the appropriate file, or download the particualr transform in an Excel wok book with both individual arrays and strain means. Please refer to the Usage Conditions and Limitations page and the References page for background on appropriate use and citations of these data.

    About the tissue used to generate these data:

Most expression data are averages based on three microarrays (U74Av2). Each individual array experiment involved a pool of brain tissue (forebrain plus the midbrain, but without the olfactory bulb) that was taken from three adult animals usually of the same age. A total of 100 arrays were used: 74 were female pools and 26 were male pools. Animals ranged in age from 56 to 441 days, usually with a balanced design (one pool at 8 weeks, one pool at ~20 weeks, one pool at approximately 1 year). you can select the strain symbol in the table above to review some details about the specific cases. You can also click on the individual symbols (males or females) to view the array image.

    About data processing:

Probe set original data from the .CHP file: These .CHP files were generated using the MAS 5.0.

    About the chromosome and megabase position values:

The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 (mm4) Assembly (see http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=mouse). We thank Dr. Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.

    About the array probe set names:

In addition to the _at (anti-sense target) and _st (sense target) probe set name designations, there are other designations that reflect special characteristics of a particular probe set based on probe design and selection crieteria. These designaions are listed below.

Probe set name designations

_f_at (sequence family): Includes probes that target identical and/or slightly polymorphic regions of different transcripts.

_s_at (similarity constraint): Probes all target common sequences found in multiple transcripts.

_g_at (common groups): Some of the probes target identical sequences and some target unique sequences regions .

_r_at (rules dropped): "Designates sequences for which it was not possible to pick a full set of unique probes using Affymetrix' probe selection rules. Probes were picked after dropping some of the selection rules."

_i_at (incomplete): "Designates sequences for which there are fewer than the required numbers of unique probes specified in the design."

Most of the descriptions for the probe set ID extensions above were taken from the Affymetrix GeneChip Expression Analysis Fundamentals.

    Data source acknowledgment:

Data were generated with funds to RWW from the Dunavant Chair of Excellence, University of Tennessee Health Science Center, Department of Pediatrics. The majority of arrays were processed at Genome Explorations by Dr. Divyen Patel.