From d0911a04958a04042da02a334ccc528dae79cc17 Mon Sep 17 00:00:00 2001
From: zsloan
Date: Fri, 27 Mar 2015 20:28:51 +0000
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the directory to 'genotype_files'
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PowerPoint Presentation - Complex trait analysis, develop-ment, and
genomics
How
to make recombinant inbred strains (RI)
C57BL/6J (B)
DBA/2J (D)
F1
20 generations brother-sister matings
BXD1
BXD2
BXD80
+ É +
F2
BXD
RI
Strain
set
fully
inbred
isogenic
hetero-
geneous
Recombined chromosomes are needed for mapping
female
male
chromosome pair
Inbred
Isogenic
siblings
BXD
The next set of slides
provide a very short interlude on QTL mapping. You will need to do some
independent reading on this topic if this is your first exposure to QTL
mapping. The recombinant inbred strains that we are using in WebQTL and in
this particular demo were generated about 25 years ago by Dr. Ben Taylor at
The Jackson Laboratory. He crossed a female C57BL/6J mouse with a male DBA/2J
mice. At the bottom of this slide we have schematized one chromosome pair
from three out of 80 BXD RI strains.The dashed vertical lines that lead to the final BXD RI lines involve
20 full sib matings (about 6 years of breeding). Some lines dieout during inbreeding. For example,
there is no extant BXD3 or BXD4 strain.