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+UCLA BXD and BXH Cartilage
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Accession number: GN204
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+PRELIMINARY SUMMARY (private data): One of a set of two expression data sets by Alfons Jake Lusis and colleagues (UCLA) generated using two sets of Recombinant Inbred strains.
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General information and background for BXD and BXH UCLA Cartilage data sets:
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+- BXD UCLA data sets
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- BXH UCLA data sets
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+All mice were maintained on a 12 h light–12 h dark cycle and fed ad libitum.
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Mice were killed at XX weeks, and tissues, including the cartilage were immediately collected and flash-frozen in liquid nitrogen.
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All procedures of housing and treatment of animals were performed in accordance with Institutional Animal Care and Use Committee regulations (UCLA).
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Array design and preparation of labeled cDNA and hybridizations to microarrays for the mouse tissue samples. RNA preparation and array hybridizations were performed at UCLA using Illumina Mouse 6 version 1.1 arrays.
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Mouse cartilage was homogenized, and total RNA was extracted using Trizol (Invitrogen) according to manufacturer's protocol. ADD MORE.
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Arrays were quantified using Illumina Rank Invariant protocol and normalized to a mean of 8 units and a SEM of all probes to 2.0 units (a modified Z score).
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Data entered by Evan Williams (July 24, 2008).
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These unapproved notes by RWW, Sept 4, 2008.
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